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Cotton Oligonucloetide Microarray

  Quality Control  
 

The spot morphology was assessed after each printed batch using Cy3-labeled, random 9-mer oligos (SpotCheck kit from Genetix)

 
Batch 1

160 slides were printed in this batch. Slide #85 was chosen at random for oligonucleotide spot quality control.

Top of slide #85

Middle of slide #85

Close-up of middle of slide #85

Another close-up of slide #85

   
 
Batch 2

172 slides were printed in this batch. Slides #32 and 151 were chosen at random for oligonucleotide spot quality control.

Top of slide #32

Close-up of top of slide #32

Top of slide #32

Close-up of top of slide #32

   
 
Batch 3

167 slides were printed in this batch. Slide #185 was chosen at random for oligonucleotide spot quality control.

Image of slide #185

Spreadsheet containing data for slide #185

   
 
Batch 4

200 slides were printed in this batch. Slide #111 was chosen at random for oligonucleotide spot quality control.

Image of slide #111

Spreadsheet containing data for slide #111

   
 
Batch 5

200 slides were printed in this batch. Slide #75 was chosen at random for oligonucleotide spot quality control.

Image of slide #75

Spreadsheet containing data for slide #75

 

 


We welcome your comments and suggestions.