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         Blast your sequences to our latest cotton assembly:
Cotton32 is an assembly of 454 reads and all the Sanger ESTs in Genbank (as of Nov 2008). It contains 1.8 million Sanger and 454 reads (G. hirsutum, G. raimondii, and G. arboreum) that are assembled into 71,568 contigs (27,951 contigs > 500 bp). Of this large assembly, ~350,000 are long Sanger reads and the remainder is composed of shorter (~200 bp) 454 FLX reads (722,578 GA, 577,375 GH, 144,236 GR). Each of the 454 FLX libraries are composed of equi-molar amounts of various cotton tissues including gynoecium, calyx, fiber, roots, whole seedlings (stem & leaf), petals, and leaves. One of the libraries (G. raimondii) had a large proportion of the cDNA library adapter embedded in >80K 454 sequences. Cotton32 used the adapter sequence as a 'vector' sequence to remove/ignore during assembly. A subsequent 454 run of G. raimondii mix RNAs is forthcoming (454 Titanium) to compensate for the low numbers of D-genome reads.

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