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Fiber Evolution

Introgression Populations
Homoeolog-specific Profiling
Genetic Networks & Phenotype
Effects of Selection
Sequence Capture

Genetic and Physical mapping resources
Comparative BAC Sequencing
Genome Sequence Resources
EST D-genome map
EST Resources

Web Database
Education and Outreach
Significance for cotton industry
Cotton Literature
Cotton Links
Wendel Lab
PGML (Paterson Lab)
Udall Lab

Lists & protocols
How to
CEGC Site Search

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Genetic and Physical mapping resources

Overview | Consensus map | People | Publications | Further Information

Further Information

For diverse purposes including rapid mapping of fiber genes, we will progress toward identifying a minimum BAC tiling path for CottonFiber. Although this goal can be fully met only by a genomic sequence, a first approximation can be made at less cost using genetic/physical approaches that have led to the production of detailed gene maps in other taxa, and which will provide a biologically-relevant scaffold upon which to hang a complete sequence in the future. With the present STS genetic map at an average of 0.8-cM intervals, transition from a genetic to a physical approach is warranted to provide better resolution and cost-efficiency. In view of the well-documented alignment of A- and D-genomes, the simplest way to accomplish this goal is to identify a minimal tiling path of BACs that collectively covers the D genome, the smaller of the two diploid genomes. Such a tiling path, re-arrayed into its physical order, can be used to hybridize large numbers of gene-specific overgo probes, quickly revealing the locations of all gene family members, independently of levels of polymorphism or other biases.

We welcome your comments and suggestions.